Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rabbits are used for polyclonal antibody production by Cloud Clone Corp. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cloud Clone Corp adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
1-Cys; AOP2; NSGPx; PRX; aiPLA2; p29; Acidic calcium-independent phospholipase A2; Antioxidant protein 2; Non-selenium glutathione peroxidase; Red blood cells page spot 12
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Avoid repeated freeze/thaw cycles. Store at 4 ℃ for frequent use. Aliquot and store at -20℃ for 12 months.
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
PBS, pH7.4,containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Applicable Secondary Antibody:
SAA544Rb59, SAA544Rb58, SAA544Rb57, SAA544Rb18, SAA544Rb19
Polyclonal antibody preparation
Polyclonals and antibodies
Rattus norvegicus (Rat)
Sequence of immunogen:
4℃ with ice bags