Properties:
If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.
Antigen background:
Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system. It is not able to receive electrons from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2).
Synonyms:
PRP; TSA; PRX2; PTX1; TPX1; NKEFB; PRXII; TDPX1; NKEF-B; HEL-S-2a; Peroxiredoxin-2; Natural killer cell-enhancing factor B; Thiol-specific antioxidant protein; Thioredoxin peroxidase 1; Thioredoxin-dependent peroxide reductase 1; PRDX2
Specificity:
This is a highly specific antibody against Peroxiredoxin 2. This antibody may have a minor secondary cross-reactivity to related protein Peroxiredoxin 1 due to a 71% non-sequential sequence similarity over the immunogen range.
Storage conditions:
Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
Crossreactive species details:
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Source:
KLH conjugated synthetic peptide derived from human Peroxiredoxin 2
Long name:
Peroxiredoxin 2 Polyclonal Antibody, FITC Conjugated
Purification method:
This antibody was purified via Protein A.
Host Organism:
Rabbit (Oryctolagus cuniculus)
Category:
Conjugated Primary Antibodies
Clone:
Polyclonal antibody
Recommended dilutions:
IF(IHC-P)(1:50-200)
Additional conjugation:
Anti-FITC Antibody
Crossreactivity:
Human, Mouse, Rat
Target Antigen:
Peroxiredoxin 2
Immunogen_range:
150-197/198
Excitation Emission:
494nm/518nm
Concentration:
1ug per 1ul
Clonality:
Polyclonal
Modification:
Unmodified
French translation:
anticorps
Tested applications:
IF(IHC-P)
Subcellular location:
Cytoplasm
Swiss Prot:
P32119
Modification site:
None
Conjugation:
FITC
Gene ID number:
7001