Test principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peroxiredoxin 5 (PRDX5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peroxiredoxin 5 (PRDX5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peroxiredoxin 5 (PRDX5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Peroxiredoxin 5 (PRDX5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
About:
Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Alternative Names:
PLP; PRDX6; ACR1; PMP20; PRXV; SBBI10; Thioredoxin Peroxidase PMP20; Peroxisomal Antioxidant Enzyme; TPx Type VI; Liver Tissue 2D-Page Spot 71B; Alu Co-Repressor 1
Properties:
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Test:
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Latin name:
Rattus norvegicus
Research Area:
Enzyme & Kinase;
Detection range:
78-5000pg/mL
Standard:
5000pg/mL
Assay Type:
Sandwich
Sensitivity:
34pg/mL
Assay length:
3h