Storage:
The lyophilized Peroxiredoxin 3 antibody can be stored at 4oC to -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. After reconstitution, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
Description:
Peroxiredoxin 3 is a mitochondrial protein belonging to the AhpC/TSA family. Expression of PRDX3 is induced by MYC and is reduced in c-myc -/- cells. Chromatin immunoprecipitation analysis spanning the entire PRDX3 genomic sequence revealed that MYC binds preferentially to a 930-bp region surrounding exon 1. Results using mitochondria-specific fluorescent probes demonstrated that Peroxiredoxin 3 is essential for maintaining mitochondrial mass and membrane potential in transformed rat and human cells.
Species reactivity:
Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
Properties:
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Notes:
Titration of the Peroxiredoxin 3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Immunogen:
Amino acids 239-256 (TIKPSPAASKEYFQKVNQ-human) were used as the immunogen for this Peroxiredoxin 3 antibody.
Intented use:
This Peroxiredoxin 3 antibodyis to be used only for research purposes and not for diagnostics..
Recommended dilutions:
Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
Concentration:
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host animal:
Rabbit (Oryctolagus cuniculus)
Clonality:
Polyclonal (rabbit origin)
Form:
Antigen affinity purified
Recognised antigen:
Peroxiredoxin 3 (PRDX3)
Clone:
Polyclonal antibody
Purity:
Antigen affinity
Tested applications:
WB, IHC-P, ICC
Conjugation:
Unconjugated
French translation:
anticorps
Category:
Antibody
Uniprot #:
P30048