Peroxiredoxin 5, also known as PRX5 and PRDX5, is a member of the peroxiredoxin family and may play an antioxidant protective role in various tissues under nonpathologic conditions and during inflammatory processes. PRDX5 displays mitochondrial presequence features and has 3 cysteines implicated in antioxidant activity and a C-terminal SQL peroxisomal targeting sequence. Northern blot analysis revealed ubiquitous expression of a 1.0-kb PRDX5 transcript in tissues and cell lines. Functional analysis showed that Peroxiredoxin 5 has antioxidant activity equivalent to that of CAT. While PRDX5 was localized to fibroblasts in normal tendon, it was localized to fibroblasts and endothelial cells in degenerative tendon. mRNA and protein levels increased at 12 hours, and the increase in PRDX5 expression correlated with reduced peroxide levels. Peroxiredoxin 5 plays a protective role against oxidative stress in human cartilage.
After reconstitution, the Peroxiredoxin 5 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
The stated application concentrations are suggested starting amounts. Titration of the Peroxiredoxin 5 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
An amino acid sequence from the C-terminus of human PRDX5 (DDSLVSIFGNRRLKRF) was used as the immunogen for this Peroxiredoxin 5 antibody (100% homologous in human, mouse and rat).
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
This Peroxiredoxin 5 antibodyis to be used only for research purposes and not for diagnostics..
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml
Rabbit (Oryctolagus cuniculus)
Polyclonal (rabbit origin)
Antigen affinity purified
Peroxiredoxin 5 (PRDX5)