Background of the antigen:
Peroxiredoxin (Prx) is an antioxidant enzyme detoxifying reactive oxygen species and has a cysteine at the active site. Prx enzymes modulate various receptor signaling pathways and protect cells from oxidatively induced death. Peroxiredoxin 1 to 4 have two conserved Cys residues corresponding to Cys51 and Cys172 of mammalian Peroxiredoxin 1. The active site cysteine(Cys51) is oxidized to cysteine sulfenic acid(Cys51-SOH) when a peroxide is reduced. Because Cys51-SOH is unstable, it forms a disulfide with Cys172-SH which comes from the other subunit of the homodimer. The disulfide is then reduced back to the Prx active thiol form by the thioredoxin-thioredoxin reductase system. However, the formation of the disulfide is a slow process. Thus under oxidative stress conditions, the sulfenic intermediate(Cys51-SOH) can be easily over oxidized to cysteine sulfinic acid(Cys-SO2H) or cysteine sulfonic acid(Cys-SO3H) before it is able to form a disulfide. Recent studies suggest that over oxidized Prx can be reduced back to the active form during recovery after oxidative stress.
Properties:
For facs or microscopy Alexa 1 conjugate.Alexa Fluor 488 has the same range to that of fluorescein isothiocyanate (FITC), yet the Anti-Peroxiredoxin 2 has a very high photo stability. As a result of this photo stability, it has turned into an antibody for fluorescent microscopy and FACS FLOW cytometry. It is distinguished in the FL1 of a FACS-Calibur or FACScan. Also Alexa Fluor 488 is pH stable.If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies. ALEXA FLUOR they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Synonyms:
MGC4104; Natural killer cell enhancing factor B; Natural killer cell-enhancing factor B; Natural Killer Enhancing Factor B; NKEF B; NKEF-B; NKEFB; Peroxiredoxin-2; PRDX 2; PRDX2; PRDX2_HUMAN; PRP; PRX2; PRXII; TDPX1; Thiol Specic Antioxidant 1; Thiol specic antioxidant protein; Thiol-specic antioxidant protein; Thioredoxin Dependent Peroxide Reductase 1; Thioredoxin Peroxidase 1; Thioredoxin-dependent peroxide reductase 1; Torin; TPX1; TSA.
Storage conditions:
Store this antibody in aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Keep refrigerated at 2 to 8 degrees Celcius for up to one year.
Source:
This antibody was obtained by immunization of the host with KLH conjugated synthetic peptide derived from human Peroxiredoxin-2
Cross-reactive species details:
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Long name:
Peroxiredoxin 2 Polyclonal Antibody, ALEXA FLUOR 488 Conjugated
Specificity:
This is a highly specific antibody against Peroxiredoxin 2.
Also known as:
Anti-Peroxiredoxin 2 PAb ALEXA FLUOR 488
Host Organism:
Rabbit (Oryctolagus cuniculus)
Category:
Conjugated Primary Antibodies
Purification:
Purified by Protein A.
Clone:
Polyclonal antibody
Recommended dilutions:
IF(IHC-P)(1:50-200)
Conjugated with:
ALEXA FLUOR® 488
Crossreactivity:
Human, Mouse, Rat
Target Antigen:
Peroxiredoxin 2
Concentration:
1ug per 1ul
Conjugation:
Alexa Fluor
Excitation emission:
499nm/519nm
Clonality:
Polyclonal
Modification:
Unmodified
French translation:
anticorps
Tested applications:
IF(IHC-P)
Modification Site:
None
Gene ID Number:
7001