Background information:
Involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system. It is not able to receive electrons from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2).
Advisory:
Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
Cross Reactive Species details:
No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
About:
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
Synonyms:
PRP; TSA; PRX2; PTX1; TPX1; NKEFB; PRXII; TDPX1; NKEF-B; HEL-S-2a; Peroxiredoxin-2; Natural killer cell-enhancing factor B; Thiol-specific antioxidant protein; Thioredoxin peroxidase 1; Thioredoxin-dependent peroxide reductase 1; PRDX2
Specificity:
This antibody may have a minor secondary cross-reactivity to related protein Peroxiredoxin 1 due to a 71% non-sequential sequence similarity over the immunogen range.Peroxiredoxin 2
Description:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Storage:
Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
Antigen Source:
KLH conjugated synthetic peptide derived from human Peroxiredoxin 2
Cross reactive species:
Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
Modification:
No modification has been applied to this antibody
Properties:
For facs or microscopy Alexa 1 conjugate.
Also known as:
Peroxiredoxin 2 Polyclonal Antibody
Other name:
Anti-Peroxiredoxin 2 Polyclonal
Host organism:
Rabbit (Oryctolagus cuniculus)
Conjugation:
Alexa Fluor,ALEXA FLUOR® 594
Type:
Conjugated Primary Antibody
Group:
Polyclonals and antibodies
Purification method:
Purified by Protein A.
Clone:
Polyclonal Antibodies
Applications with corresponding dilutions:
IF(IHC-P)(1:50-200)
Clonality:
Polyclonal Antibody
Conjugated:
Alexa conjugate 1
Conjugated with:
ALEXA FLUOR® 594
Target Protein/Peptide:
Peroxiredoxin 2
Excitation emission:
590nm/617nm
Concentration:
1ug per 1ul
Applications:
IF(IHC-P)
Subcellular locations:
Cytoplasm
Swiss Prot:
P32119
Modification site:
None
Gene ID:
7001