Background information:
Peroxiredoxin (Prx) is an antioxidant enzyme detoxifying reactive oxygen species and has a cysteine at the active site. Prx enzymes modulate various receptor signaling pathways and protect cells from oxidatively induced death. Peroxiredoxin 1 to 4 have two conserved Cys residues corresponding to Cys51 and Cys172 of mammalian Peroxiredoxin 1. The active site cysteine(Cys51) is oxidized to cysteine sulfenic acid(Cys51-SOH) when a peroxide is reduced. Because Cys51-SOH is unstable, it forms a disulfide with Cys172-SH which comes from the other subunit of the homodimer. The disulfide is then reduced back to the Prx active thiol form by the thioredoxin-thioredoxin reductase system. However, the formation of the disulfide is a slow process. Thus under oxidative stress conditions, the sulfenic intermediate(Cys51-SOH) can be easily over oxidized to cysteine sulfinic acid(Cys-SO2H) or cysteine sulfonic acid(Cys-SO3H) before it is able to form a disulfide. Recent studies suggest that over oxidized Prx can be reduced back to the active form during recovery after oxidative stress.
Synonyms:
MGC4104; Natural killer cell enhancing factor B; Natural killer cell-enhancing factor B; Natural Killer Enhancing Factor B; NKEF B; NKEF-B; NKEFB; Peroxiredoxin-2; PRDX 2; PRDX2; PRDX2_HUMAN; PRP; PRX2; PRXII; TDPX1; Thiol Specic Antioxidant 1; Thiol specic antioxidant protein; Thiol-specic antioxidant protein; Thioredoxin Dependent Peroxide Reductase 1; Thioredoxin Peroxidase 1; Thioredoxin-dependent peroxide reductase 1; Torin; TPX1; TSA.
Advisory:
Avoid freeze/thaw cycles as they may denaturate the polypeptide chains of the antibody, thus reducing its reactivity, specificity and sensitivity. For antibodies that are in liquid form or reconstituted lyophilized antibodies small amounts could become entrapped on the seal or the walls of the tube. Prior to use briefly centrifuge the vial to gather all the solution on the bottom.
Cross Reactive Species details:
No significant cross reactivity has been observed for this antibody for the tested species. However, note that due to limited knowledge it is impossible to predict with 100% guarantee that the antibody does not corss react with any other species.
About:
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
Description:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Storage:
Water buffered solution containing 100ug/ml BSA, 50% glycerol and 0.09% sodium azide. Store at 4°C for 12 months.
Antigen Source:
KLH conjugated synthetic peptide derived from human Peroxiredoxin-2
Cross reactive species:
Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus)
Specificity:
This antibody reacts specifically with Peroxiredoxin 2
Modification:
No modification has been applied to this antibody
Properties:
For facs or microscopy Alexa 1 conjugate.
Also known as:
Peroxiredoxin 2 Polyclonal Antibody
Other name:
Anti-Peroxiredoxin 2 Polyclonal
Host organism:
Rabbit (Oryctolagus cuniculus)
Conjugation:
Alexa Fluor,ALEXA FLUOR® 594
Type:
Conjugated Primary Antibody
Group:
Polyclonals and antibodies
Purification method:
Purified by Protein A.
Clone:
Polyclonal Antibodies
Clonality:
Polyclonal Antibody
Applications with corresponding dilutions:
IF(IHC-P)(1:50-200)
Conjugated with:
ALEXA FLUOR® 594
Conjugated:
Alexa conjugate 1
Target Protein/Peptide:
Peroxiredoxin 2
Excitation emission:
590nm/617nm
Concentration:
1ug per 1ul
Applications:
IF(IHC-P)
Modification site:
None
Gene ID:
7001
Subcellular locations:
N/A
Swiss Prot:
N/A