References:
Yoshida et al. (2015). Thioredoxin Selectivity for Thiol-Based Redox Regulation of Target Proteins in Chloroplasts. J Biol Chem. 2015 Apr 15. pii: jbc.M115.647545. Feifei et al. (2014). Comparison of Leaf Proteomes of Cassava (Manihot esculenta Crantz) Cultivar NZ199 Diploid and Autotetraploid Genotypes. PLoS One. 2014 Apr 11;9(4):e85991. doi: 10.1371/journal.pone.0085991. eCollection 2014. Wu et al. (2013). Proteomic and Phytohormone Analysis of the Response of Maize (Zea mays L.) Seedlings to Sugarcane Mosaic Virus. PLoS One. July 23;8(7).
Scientific context:
Peroxiredoxins (EC=1.11.1.15) belong to the enzyme family which is ubiquitous in all kingdoms of life. Prx Q enzyme acting by reducing hydroperoxides. Peroxiredoxins have no heme group, unlike the other peroxidases, but perform their enzymatic activity using cysteine residues with redox-active thiol groups. The ability of peroxiredoxins to hydrolyze hydroperoxides suggests that this protein family has a general function in oxidant defence.
Storage condition:
store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Immunogen:
His-tagged full length protein (with presequence) of Arabidopsis thaliana was overexpressed in in E.coli. Isolated with HiTrap column (GE Healthcare) Q9LU86, At3g26060
Supplementary information:
In stroma fractions a weak background reaction at 28 kDa is visible. No crossreactivity in any thylakoid fractions.
No reactivity:
no confirmed exceptions from predicted reactivity known in the moment
Verified reactivity:
Arabidopsis thaliana, Manihot esculenta, Spinacia oleracea, Zea mays
Possible reactivity:
Populus spp. , monocots including Triticum aestivum, Oryza sativa
Connected products:
Plant protein extraction buffer Secondary antibodies
How to reconstitute:
For reconstitution add 200 µl of sterile water.
Recommended dilutions for use:
1: 5000 with standard ECL (WB)
Verified applications:
western blot (WB)
Available ordering format:
Lyophilized
Clone:
Polyclonal
Clonality:
Polyclonal
TAIR number:
AT3G26060
Molecular weight (expected | аpparent):
16 kDA
Raised in:
Rabbit
Protein number:
Q9LU86
Purification:
Serum
Notes:
None