Human Peroxiredoxin 2 PicoKine ELISA Kit

Basic

Information

Name

Human Peroxiredoxin 2 PicoKine ELISA Kit

Size

1x96 well plate

Catalog number

EK1528

Price

557 EUR

Extended

Details

More gene info:
Peroxiredoxin-2 is a protein that in humans is encoded by the PRDX2 gene. This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein plays an antioxidant protective role in cells, and it may contribute to the antiviral activity of CD8(+) T-cells. The crystal structure of this protein has been resolved to 2.7 angstroms. This protein prevents hemolytic anemia from oxidative stress by stabilizing hemoglobin, thus making this gene a therapeutic target for patients with hemolytic anemia. It may have a proliferative effect and play a role in cancer development or progression. Related pseudogenes have been identified on chromosomes 5, 6, 10 and 13.
ELISA kit description:
GENTAUR's human Peroxiredoxin 2 ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for Peroxiredoxin 2 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: A2-N198) and your samples are measured with a biotin conjugated polyclonal from goat specific for Peroxiredoxin 2 will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more human Peroxiredoxin 2 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
How the elisa works:
Sample the amount of 100ul/well of the standard solution at 20000pg/ml, 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml human Peroxiredoxin 2 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatant, cell lysate, tissue homogenates, serum or plasma (heparin, EDTA) to the ELISA plate. Keep in mind that each human Peroxiredoxin 2 standard solution and each sample be measured in duplicate.
The ELISA contains:
Catalog number;Description;Quantity -;96-well plate precoated with anti- Human Peroxiredoxin 2 antibodies;1 ST0000-20;Lyophilized recombinant human Peroxiredoxin 2 standard;20ng/tube×2 AR1107;Biotinylated anti- human Peroxiredoxin 2 antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
Properties:
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
ELISA's precision parameters:
intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (ng/ml);3.45;7.98;13.73;4.20;9.27;15.76 ELISA standard deviations 0.15;0.44;0.95;0.24;0.58;1.13 CV(%);4.3;5.5;6.9;5.8;6.3;7.2
Alternative:
NKEFB ; NKEF-B ; NKEFB ; TDPX1 ; Peroxiredoxin 2 ; Peroxiredoxin2 ; Peroxiredoxin-2 ; PRDX2 ; PRX2 ; Thiol-specific antioxidant protein ; TSA ; P32119
ELISA's application:
For quantitative measurement of human Peroxiredoxin 2 in cell culture supernatant, cell lysate, tissue homogenates, serum and plasma heparin, EDTA .
ELISA standard curve:
Concentration(pg/ml) + 0 + 312 + 625 + 1250 + 2500 + 5000 + 10000 + 20000 O.D. + 0.030 + 0.061 + 0.106 + 0.175 + 0.423 + 0.764 + 1.301 + 2.194
Keep at:
Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Cross reaction:
the ELISA does not cross react with any other proteins except from closely related species
Detector and capure antibodies:
a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat
Test:
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Target of this ELISA:
Natural and recombinant human Peroxiredoxin 2
ELISA kit detection limits:
312pg/ml to 20000pg/ml
Gene tested:
human Peroxiredoxin 2
Anti-coagulation:
heparin or EDTA
Uniprot:
Peroxiredoxin-2
Gene name:
peroxiredoxin 2
Antigen:
NSO, A2-N198
ELISA's sensitivity:
<10pg/ml
Gen protein identity:
P32119
Minutes of TMB incubation:
25-30
Entrez nr.:
7001