Antigen background:
Peroxiredoxin (Prx) is an antioxidant enzyme detoxifying reactive oxygen species and has a cysteine at the active site. Prx enzymes modulate various receptor signaling pathways and protect cells from oxidatively induced death. Peroxiredoxin 1 to 4 have two conserved Cys residues corresponding to Cys51 and Cys172 of mammalian Peroxiredoxin 1. The active site cysteine(Cys51) is oxidized to cysteine sulfenic acid(Cys51-SOH) when a peroxide is reduced. Because Cys51-SOH is unstable, it forms a disulfide with Cys172-SH which comes from the other subunit of the homodimer. The disulfide is then reduced back to the Prx active thiol form by the thioredoxin-thioredoxin reductase system. However, the formation of the disulfide is a slow process. Thus under oxidative stress conditions, the sulfenic intermediate(Cys51-SOH) can be easily over oxidized to cysteine sulfinic acid(Cys-SO2H) or cysteine sulfonic acid(Cys-SO3H) before it is able to form a disulfide. Recent studies suggest that over oxidized Prx can be reduced back to the active form during recovery after oxidative stress.
Synonyms:
MGC4104; Natural killer cell enhancing factor B; Natural killer cell-enhancing factor B; Natural Killer Enhancing Factor B; NKEF B; NKEF-B; NKEFB; Peroxiredoxin-2; PRDX 2; PRDX2; PRDX2_HUMAN; PRP; PRX2; PRXII; TDPX1; Thiol Specic Antioxidant 1; Thiol specic antioxidant protein; Thiol-specic antioxidant protein; Thioredoxin Dependent Peroxide Reductase 1; Thioredoxin Peroxidase 1; Thioredoxin-dependent peroxide reductase 1; Torin; TPX1; TSA.
Storage conditions:
Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
Properties:
If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Crossreactive species details:
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Conjugated:
These antibodies are excite for emission at 650 nm and detected at a 676 nm wavelengths.
Source:
KLH conjugated synthetic peptide derived from human Peroxiredoxin-2
Specificity:
This is a highly specific antibody against Peroxiredoxin 2.
Long name:
Peroxiredoxin 2 Polyclonal Antibody, Cy7 Conjugated
Purification method:
This antibody was purified via Protein A.
Host Organism:
Rabbit (Oryctolagus cuniculus)
Category:
Conjugated Primary Antibodies
Clone:
Polyclonal antibody
Recommended dilutions:
IF(IHC-P)(1:50-200)
Crossreactivity:
Human, Mouse, Rat
Target Antigen:
Peroxiredoxin 2
Excitation Emission:
743nm/767nm
Concentration:
1ug per 1ul
Clonality:
Polyclonal
Modification:
Unmodified
Tested applications:
IF(IHC-P)
French translation:
anticorps
Gene ID number:
7001
Modification site:
None
Conjugation:
Cy7
Additional conjugation:
Cy7