Antigen background:
Peroxiredoxin (Prx) is an antioxidant enzyme detoxifying reactive oxygen species and has a cysteine at the active site. Prx enzymes modulate various receptor signaling pathways and protect cells from oxidatively induced death. Peroxiredoxin 1 to 4 have two conserved Cys residues corresponding to Cys51 and Cys172 of mammalian Peroxiredoxin 1. The active site cysteine(Cys51) is oxidized to cysteine sulfenic acid(Cys51-SOH) when a peroxide is reduced. Because Cys51-SOH is unstable, it forms a disulfide with Cys172-SH which comes from the other subunit of the homodimer. The disulfide is then reduced back to the Prx active thiol form by the thioredoxin-thioredoxin reductase system. However, the formation of the disulfide is a slow process. Thus under oxidative stress conditions, the sulfenic intermediate(Cys51-SOH) can be easily over oxidized to cysteine sulfinic acid(Cys-SO2H) or cysteine sulfonic acid(Cys-SO3H) before it is able to form a disulfide. Recent studies suggest that over oxidized Prx can be reduced back to the active form during recovery after oxidative stress.
Properties:
If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser. 1 of the Anti-Peroxiredoxin 2 can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC. This Bioss Primary Conjugated Antibodies antibody is well suited for fluorescent microscopy.
Synonyms:
MGC4104; Natural killer cell enhancing factor B; Natural killer cell-enhancing factor B; Natural Killer Enhancing Factor B; NKEF B; NKEF-B; NKEFB; Peroxiredoxin-2; PRDX 2; PRDX2; PRDX2_HUMAN; PRP; PRX2; PRXII; TDPX1; Thiol Specic Antioxidant 1; Thiol specic antioxidant protein; Thiol-specic antioxidant protein; Thioredoxin Dependent Peroxide Reductase 1; Thioredoxin Peroxidase 1; Thioredoxin-dependent peroxide reductase 1; Torin; TPX1; TSA.
Storage conditions:
Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
Crossreactive species details:
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Source:
KLH conjugated synthetic peptide derived from human Peroxiredoxin-2
Specificity:
This is a highly specific antibody against Peroxiredoxin 2.
Long name:
Peroxiredoxin 2 Polyclonal Antibody, Cy3 Conjugated
Purification method:
This antibody was purified via Protein A.
Host Organism:
Rabbit (Oryctolagus cuniculus)
Category:
Conjugated Primary Antibodies
Clone:
Polyclonal antibody
Excitation Emission:
512,550nm/570,615nm
Recommended dilutions:
IF(IHC-P)(1:50-200)
Additional conjugation:
cy3 conjugation kit
Crossreactivity:
Human, Mouse, Rat
Target Antigen:
Peroxiredoxin 2
Concentration:
1ug per 1ul
Clonality:
Polyclonal
Modification:
Unmodified
French translation:
anticorps
Tested applications:
IF(IHC-P)
Modification site:
None
Gene ID number:
7001
Conjugation:
Cy3