Background information::
Peroxiredoxin (Prx) is a growing peroxidase family, whose mammalian members have been known to connect with cell proliferation, differentiation, and apoptosis. Many isoforms (about 50 proteins), collected in accordance to the amino acid sequence homology, particularly amino-terminal region containing active site cysteine residue, and the thiol-specific antioxidant activity, distribute throughout all the kingdoms. Among them, mammalian Prx consists of 6 different members grouped into typical 2-Cys, atypical 2-Cys Prx, and 1-Cys Prx. Except Prx VI belonging to 1-Cys Prx subgroup, the other five 2-Cys Prx isotypes have the thioredoxin-dependent peroxidase (TPx) activity utilizing thioredoxin, thioredoxin reductase, and NADPH as a reducing system. Mammalian Prxs are 20 – 30 kDa in molecular size and vary in subcellular localization: Prx I, II, and VI in cytosol, Prx III in mitochondria, Prx IV in ER and secretion, Prx V showing complicated distribution including peroxisome, mitochondria and cytosol. Prx II is involved in redox regulation of the cell. Reduces peroxides with reducing equivalents provided through the thioredoxin system. It is not able to receive electrons from glutaredoxin. May play an important role in eliminating peroxides generated during metabolism. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H2O2.
Description:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties:
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Formulation of this antibody::
100 µl of antibody in HEPES with 0.15 M NaCl, 0.01 % BSA, 0.03 % sodium azide, and 50 % glycerol
Alternative names of antibody target::
Peroxiredoxin 2, NKEFB, PRP, PRX2, PRXII, TDPX1, TSA, PRDX2.
How to handle this antibody::
The antibody solution should be gently mixed before use.
Purification method::
Ammonium sulphate precipitation purified antibody
Immunogene sequence::
Recombinant human protein purified from E.coli
Recommended usage::
Western blot: 1:2000 – 1:4000, ELISA, IHC-P.
Storage information::
Shelf time: 12 months in temp.: -20 deg.C
This antibody has been succesfully tested for use in following methods::
Western blot,ELISA, IHC-P.
This antibody reacts with::
Human, Mouse, Rat
Target of this antibody::
Peroxiredoxin II
Isotype::
Mouse IgG 1
Clonality of antibody::
Monoclonal
French translation:
anticorps
Shipping conditions::
gel pack
Concentration of antibody::
1 mg/ml
Form of antibody::
Liquid
Antibody host::
Mouse
Clone::
1E8