Background information::
Peroxiredoxin (Prx) is a growing peroxidase family, whose mammalian members have been known to connect with cell proliferation, differentiation, and apoptosis. Many isoforms (about 50 proteins), collected in accordance to the amino acid sequence homology, particularly amino-terminal region containing active site cysteine residue, and the thiol-specific antioxidant activity, distribute throughout all the kingdoms. Among them, mammalian Prx consists of 6 different members grouped into typical 2-Cys, atypical 2-Cys Prx, and 1-Cys Prx. Except Prx VI belonging to 1-Cys Prx subgroup, the other five 2-Cys Prx isotypes have the thioredoxin-dependent peroxidase (TPx) activity utilizing thioredoxin, thioredoxin reductase, and NADPH as a reducing system. Mammalian Prxs are 20 – 30 kDa in molecular size and vary in subcellular localization: Prx I, II, and VI in cytosol, Prx III in mitochondria, Prx IV in ER and secretion, Prx V showing complicated distribution including peroxisome, mitochondria and cytosol. Prx IV is probably involved in redox regulation of the cell. Regulates the activation of NFkappaB in the cytosol by a modulation of I-kappa-B-alpha phosphorylation.
Description:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties:
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Formulation of this antibody::
100 µl of antibody in HEPES with 0.15 M NaCl, 0.01 % BSA, 0.03 % sodium azide, and 50 % glycerol
Immunogene sequence::
Recombinant human fragment protein (without secretion leader sequence) purified from E.coli.
How to handle this antibody::
The antibody solution should be gently mixed before use.
Recommended usage::
Western blot: 2-4 µl/ml, IP: 1-2 µl, ICC: 20 µl/ml.
Purification method::
Ammonium sulphate precipitation purified antibody
Storage information::
Shelf time: 12 months in temp.: -20 deg.C
Alternative names of antibody target::
Peroxiredoxin 4, PRX-4, AOE37-2, PRDX4.
This antibody has been succesfully tested for use in following methods::
Western blot,IP,ICC
Target of this antibody::
Peroxiredoxin IV
Isotype::
Mouse IgG 1
Clonality of antibody::
Monoclonal
French translation:
anticorps
Shipping conditions::
gel pack
Concentration of antibody::
1 mg/ml
Form of antibody::
Liquid
This antibody reacts with::
Human
Antibody host::
Mouse
Clone::
3A1