Background information::
Peroxiredoxin (Prx) is a growing peroxidase family, whose mammalian members have been known to connect with cell proliferation, differentiation, and apoptosis. Many isoforms (about 50 proteins), collected in accordance to the amino acid sequence homology, particularly amino-terminal region containing active site cysteine residue, and the thiol-specific antioxidant activity, distribute throughout all the kingdoms. Among them, mammalian Prx consists of 6 different members grouped into typical 2-Cys, atypical 2-Cys Prx, and 1-Cys Prx. Except Prx VI belonging to 1-Cys Prx subgroup, the other five 2-Cys Prx isotypes have the thioredoxin-dependent peroxidase (TPx) activity utilizing thioredoxin, thioredoxin reductase, and NADPH as a reducing system. Mammalian Prxs are 20 – 30 kDa in molecular size and vary in subcellular localization: Prx I, II, and VI in cytosol, Prx III in mitochondria, Prx IV in ER and secretion, Prx V showing complicated distribution including peroxisome, mitochondria and cytosol. Prx V is involved in reduction of hydrogen peroxide and alkyl hydroperoxides with reducing equivalents provided through the thioredoxin system. Involved in intracellular redox signaling.
Description:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties:
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Immunogene sequence::
Recombinant human Prx V fragment protein (without mitochondrial leader sequence) purified from E.coli.
Formulation of this antibody::
100 µl of antibody in HEPES with 0.15 M NaCl, 0.01 % BSA, 0.03 % sodium azide, and 50 % glycerol
Alternative names of antibody target::
Peroxiredoxin 5, ACR1, AOEB116, B116, PLP, PMP20, PRDX6, PRXV, SBBI10.
Recommended usage::
Western blot: 1:1000, IP: 1-2 µl, ICC: 20 µl/ml, ELISA.
How to handle this antibody::
The antibody solution should be gently mixed before use.
Purification method::
Ammonium sulphate precipitation purified antibody
Storage information::
Shelf time: 12 months in temp.: -20 deg.C
This antibody has been succesfully tested for use in following methods::
Western blot,IP,ICC,ELISA.
Target of this antibody::
Peroxiredoxin V
Isotype::
Mouse IgG 1
Clonality of antibody::
Monoclonal
French translation:
anticorps
Shipping conditions::
gel pack
Concentration of antibody::
1 mg/ml
Form of antibody::
Liquid
This antibody reacts with::
Human
Antibody host::
Mouse
Clone::
3F11