Test Principle:
This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human PRDX1 . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human PRDX1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human PRDX1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human PRDX1. The concentration of Human PRDX1 in samples can be calculated by comparing the OD of the samples to the standard curve.
Properties:
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Precaution of Use:
The Stop Solution is acidic. Do not allow to contact skin or eyes.
Cross Activity:
No significant cross-reactivity or interference was observed.
Test:
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Shelf life:
Use Human PRDX1 (Peroxiredoxin 1) ELISA Kit before 6 months
Quality Systems:
The kit is manufactured at ISO 9001 certified facilities.
Application:
For research use only. Not for diagnostic procedures.
Research Area:
Cancer, Cell Biology, Cardiovascular, Metabolism
Storage Temperature:
Short term: 4°C; Long term: see manual.
Sample Type:
Serum, Plasma, Biological Fluids
Estimated Turnaround Time:
6-11 business days
Detection Range:
15.63-1000 pg/mL
Detection Method:
Colorimetric
Sensitivity:
9.38 pg/mL
Assay Time:
4.5 hours
Shipping Conditions:
Ice packs
Assay Type:
Sandwich
Species Reactivity:
Human
Target's alterntive name:
PRDX1
Target Name:
NA
Specificity:
NA
Precision:
NA
Stability:
NA