Gene info:
PRDX3 is a protein with antioxidant function and is localized in the mitochondrion. Expression of this gene product in E. coli deficient in the C22-subunit gene rescued resistance of the bacteria to alkylhydroperoxide. The human and mouse genes are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Sequence comparisons with recently cloned mammalian homologues suggest that these genes consist of a family that is responsible for regulation of cellular proliferation, differentiation, and antioxidant functions. This gene encodes a protein with antioxidant function and is localized in the mitochondrion. This gene shows significant nucleotide sequence similarity to the gene coding for the C22 subunit of Salmonella typhimurium alkylhydroperoxide reductase. Expression of this gene product in E. coli deficient in the C22-subunit gene rescued resistance of the bacteria to alkylhydroperoxide. The human and mouse genes are highly conserved, and they map to the regions syntenic between mouse and human chromosomes. Sequence comparisons with recently cloned mammalian homologues suggest that these genes consist of a family that is responsible for regulation of cellular proliferation, differentiation, and antioxidant functions. Two transcript variants encoding two different isoforms have been found for this gene.
Antibody advice:
PRDX3 antibody can be used for detection of PRDX3 by ELISA at 1:312500. PRDX3 antibody can be used for detection of PRDX3 by western blot at 0.5 ug/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Keep at:
For short periods of storage (days) store at 4 grades C. For longer periods of storage, store PRDX3 antibody at -20 grades C. As with any antibody avoid repeat freeze-thaw cycles.
Description:
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Properties:
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Suspention, pH, azide:
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.
Antigen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PRDX3.
Gene names:
PRDX3, AOP-1, AOP1, MER5, MGC104387, MGC24293, PRO1748, SP-22, HBC189, prx-III
Purity:
Antibody is purified by peptide affinity chromatography method.
Immuno application:
ELISA, Western Blot
Antibodie's label:
unlabelled serum
Antigene name:
peroxiredoxin 3
Source:
Homo sapiens
Vial with antibody:
Lyophilized
Type:
Polyclonal
French translation:
anticorps
Ncbi nr:
NP_054817
Omim nr:
32483377
Quantity per ml:
1 mg/ml
KDa:
26 kDa
Protein nr:
P30048
Ab raised in:
Rabbit
NCBI GENE nr:
10935
GENE symbol:
PRDX3
Works with:
Human