Peroxiredoxin 3, also known as AOP-1, MER5, SP-22 or PRX3, is localized exclusively in mitochondria. The deduced 256-amino acid human protein shares 86% amino acid sequence similarity with the mouse protein, and significant similarity with both the human proliferation-associated gene A product and the mouse stress-induced peritoneal macrophage protein Msp23. The PRDX3 gene is mapped on 10q26.11. Expression of PRDX3 is induced by MYC and is reduced in c-myc -/- cells. Chromatin immunoprecipitation analysis spanning the entire PRDX3 genomic sequence revealed that MYC binds preferentially to a 930-bp region surrounding exon 1. Results using mitochondria-specific fluorescent probes demonstrated that PRDX3 is essential for maintaining mitochondrial mass and membrane potential in transformed rat and human cells. These data provided evidence that Peroxiredoxin 3 is a MYC target gene that is required to maintain normal mitochondrial function.
After reconstitution, the Peroxiredoxin 3 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
The stated application concentrations are suggested starting amounts. Titration of the Peroxiredoxin 3 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
An amino acid sequence from the C-terminus of mouse PRDX3 (TIKPSPTASKEYFEKVHQ) was used as the immunogen for this Peroxiredoxin 3 antibody (100% rat homology).
If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
This Peroxiredoxin 3 antibodyis to be used only for research purposes and not for diagnostics..
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml
Rabbit (Oryctolagus cuniculus)
Polyclonal (rabbit origin)
Antigen affinity purified