Summary of the prodecure:
1. Prepare the reagents and samples according to the manual; 2. Leave one well empy; 3. Place 50ul of standard or sample in each well; 4. Add 50ul of the antibody to each well except the Blank (empty); 5 Allow 60 minutes for incubation at 37 degrees celsius; 6. Wash the wells 5 times; 7. Add 100ul of the conjugate to each well but the Blank one; 8. Allow 30 more minutes for incubation at 37C; 9. Wash 5 more times; 10. Add 50ul of both Substrate A and Substrate B in each well (incl. the Blank) and incubate for 15 minutes; 11. Put 50 ul of Stop Solution in all wells (incl. the Blank) and read the results within maximum 10 minutes; Notes: *Samples might require dilution, follow the sample dilution instructions of the manual. **During the last incubation with Substrates A and B the setting should be protected from any light.
Tips:
Due to transportation and handling small amounts of the liquid components of the Homo sapiens Peroxiredoxin-5, mitochondrial ELISA Kit can get caught on the walls or lid of the vial. Prior to use be sure to briefly centrifuge all vials to ensure that all of the content is on the bottom. Do not freeze the kit, as this might denaturate the standards and the peptide chains of the antibodies used for the production if the ELISA kit. Substrate A and Substrate B are light sensitive, therefore you should protect the kits from direct light. When using the vials of Substrates A and B you may cover them in aluminium foil to protect them from light influences.
Properties:
E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Kit contents:
ELISA test kit for Human Peroxiredoxin-5, mitochondrialAssay plate 1 (96wells); Standard 5 x 0.5 ml; A ntibody 1x5ml; Conjugate 1x10ml; Wash buffer 20ml (25X); Substrate A 1x5ml; Substrate B 1x5ml; Stop solution 1x5ml;Adhesive strips (4pcs); working manual
Shipping, storage, shelf life:
The Peroxiredoxin-5, mitochondrial ELISA kit is shipped on ice packs. Upon delivery store the kit at +4 C. The shelf life of the Human Peroxiredoxin-5, mitochondrial ELISA Kit is 6 months after delivery.
Sample types:
Our Peroxiredoxin-5, mitochondrial ELISA kit has been proven to work and successfully detect Peroxiredoxin-5, mitochondrial in Human
General description:
This is a ready to use kit for performing ELISA tests for detection of Peroxiredoxin-5, mitochondrial in samples from Human
Test:
ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Long name:
ELISA test kit for Human Peroxiredoxin-5, mitochondrial
Synonym name:
Homo sapiens Peroxiredoxin-5, mitochondrial ELISA Kit
Applications:
Enzyme Linked Immunosorbent Assay
Antigen:
Peroxiredoxin-5, mitochondrial
Tissue:
mitochondrial
Detection wavelengt:
450 nm
Species reactivity:
Human
Assay procedure:
1-4h