About:
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
Usage:
Western blot (WB) analysis of PRDX5 pAb at 1:500 dilution Lane1:The Testis tissue lysate of Rat(40ug) Lane2:The Testis tissue lysate of Mouse(40ug) Lane3:SGC7901 whole cell lysate(40ug)
Purification&Purity:
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Alternative Name:
Spectrin alpha chain, non-erythrocytic 1; Alpha-II spectrin; Fodrin alpha chain; Spectrin, non-erythroid alpha subunit; SPTAN1; NEAS; SPTA2
Properties:
If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Immunogen:
Synthetic peptide, corresponding to amino acids 1155-1215 of Human Cleaved-Spectrin α II.
Storage&Stability:
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Product:
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Note:
For research use only, not for use in diagnostic procedure.
Specificity:
PRDX5 pAb detects endogenous levels of PRDX5 protein.
Group:
Polyclonals and antibodies
Application_all:
WB: 1:500~1:1000
Reactivity:
Human,Mouse,Rat
French translation:
anticorps
BiowMW:
~ 25 kDa
Swiss-Prot:
P30044
Applications:
WB